ISOLATION AND PURIFICATION OF TYROSINASE FROM DIFFERENT PLANT SOURCES
POLINA VELICHKOVA1, DESISLAVA MARINKOVA1, SPASKA YANEVA2*, LYUBOV YOTOVA1
1 – University of Chemical Technology and Metallurgy, Department of Biotechnology, Sofia, Bulgaria
2 – University of Chemical Technology and Metallurgy, Department of Bases of Chemical Technology, Sofia, Bulgaria
*Corresponding author: sp_yaneva@uctm.edu
Keywords: enzymes, tyrosinase sources, isolation, purification
Abstract: Tyrosinases from potato (Solanum tuberosum), banana (Musa acuminata) and Agaricus bisporus were isolated by two methods. The obtained filtrates were purified by precipitation with ammonium sulfate and ice-cold ethanol.
The aim of this study was to compare specific activities of enzymes, laboratory isolated from different sources using industrial purified tyrosinase as a reference.
The temperature, pH optimums and kinetic parameters were determined.