Софийски университет "Св. Климент Охридски"

Role: professor
Laboratory of Virology, Faculty of Biology

Name: Stoyan Shishkov, Ph.D.

Date and Place of Birth: 09.12.1960, Sofia, Bulgaria

Address: University of Sofia "St. Kl. Ohridski", 8 Dragan Tzankov blvd., Sofia 1164, BULGARIA

E-mail: sashishkov@yahoo.com; sshishkov@biofac.uni-sofia.bg

Phone: +359-2-8167294

Education and Training

• Sofia University, Faculty of Biology, MSc in Virology, 1985
• Institute of Microbiology, Bulgarian Academy of Sciences, Ph.D. 1991
• Institute of microbiology, Padua, Italy, 6 month, researcher, 1996.

Academic positions

• Dean of Faculty of Biology, from 2016 - present
• Head of Laboratory of Virology , from 2010 – present
• Member of the scientific council of an Institute of microbiology, Bulgarian Academy of Sciences, from 2016 – present.

Scientific Activities

Stoyan Shishkov is a professor at Sofia University "St. Kliment Ohridski", Faculty of Biology. He teaches molecular virology at the Bachelor's degree. In the master's degree, he lectures on viral replication, medical virology, therapy and prevention of viral diseases. He is the supervisor of PhD students in virology. His main scientific activity is related to the screening of substances of natural origin and those synthesized by molecular design. He participates in conducting molecular diagnostics of viral infections in humans. Another area of scientific interest is the inactivation of viruses by means of low-temperature plasma. Leads the development of a methodology for testing medical masks to protect against virus infection in the event of the coronavirus pandemic in the absence of international standards. All these activities have resulted in over 70 publications in peer-reviewed journals. He holds two national patents for antiviral substances.

Selected Publications

1. Shishkova, K., Stoymirska, A., Chayrov, R.; Shishkov, S., Sbirkova-Dimitrova, H., Rusew, R., Shivachev, B., Stankova, I. 2023. Amantadine and Rimantadine Analogues—Single-Crystal Analysis and Anti-Coronaviral Activity. Crystals, 13, 1374. https://doi.org/10.3390/cryst13091374.
2. Shishkova, K.; Gergova, R.; Tasheva, E.; Shishkov, S.; Sirakov, I. Molecular Screening for High-Risk Human Papillomaviruses in Patients with Periodontitis. Viruses 2023, 15, 809.
3. Shishkova, K., I. Tsekov, R. Popov, S. Shishkov, and Z. Kalvatchev. 2022. Research of the Genetic Diversity of TT Viruses in Bulgaria. C. R. Acad. Bulg. Sci. ,. 75, 11, 1680–1686
4. Tsvetkov, V., A. Hinkov, D. Todorov, E. Benova, I. Tsonev, T. Bogdanov, S. Shishkov & K. Shishkova. 2020. Effect of Plasma-Activated Medium and Water on Replication and Extracellular Virions of Herpes Simplex Virus-1. Plasma medicine, 10, 1, 15-26.
5. Todorov, D., Tsvetkov, V., Hinkov, A., Shishkova, K., Shishkov, S., Pavlova, D., & Mantovska, D. (2022). Effect of the soil type over the antiherpes activities of Teucrium chamaedrys. Fresenius environmental bulletin, 31(9), 9831-9838.
6. Tsvetkov, D. Todorov, A. Hinkov, K. Shishkova, L. Velkova, A. Dolashki, P. Dolashka, S. Shishkov. 2021. Effect of extracts of some species from Phylum Mollusca against the replication of Human Alphaherpesviruses. Bulgarian Chemical Communications, Volume 53, Special Issue-A, 66 – 72.
7. Hinkov A., Angelova P., Marchev A., Hodzhev Y., Tsvetkov V.,Dragolova D, Todorov D, Shishkova K., Kapchina-Toteva V., Blundell R., Shishkov S., Georgiev. M., 2020. Nepeta nuda ssp. nuda L. water extract: Inhibition of replication of some strains of Human Alpha herpes virus (genus Simplex virus) in vitro, mode of action and NMR-based metabolomics, Journal of Herbal Medicine 21, 100334.
8. Alexandrova. R., A. Aludalleh, T. Zhivkova, L. Dyakova, S. Shishkov, M. Alexandrov, G. Marinesko, D. Culita, L. Patron. 2014. Cytotoxic effect of Zn/Ag complex on cultured non-tumor cells. In: Dushovski, C., J. Radenkova-Saeva. (eds.). “Toxicological problems”, Military Publishing House Ltd., Sofia, Bulgaria, 32: 248-286.
9. Hinkov, A., H. Valchovski, S. Shishkov, K. Nedyalkova, R. Donkova. 2016. Investigation of the Antiviral and Antibacterial Potential of Coelomic Fluid from Some Earthworm Species (Oligochaeta: Lumbricidae). Acta Microbiologica Bulgarica, 32 (3): 39-44.
10. Voleva, S., Manolov, V., Krumova, S., Marinov, B., Vasilev, V., Shishkov, S., & Nikolaeva-Glomb, L. (2019). Clinical Case of Parvovirus B19 Infection in Pregnant Woman with Β-Thalassemia in Bulgaria. Clinical laboratory, 65(5).
11. Angelova, P., A. Hinkov, V. Tsvetkov, D.Todorov, K. Shishkova, D. Dragolova, S. Shishkov, V.Kapchina-Toteva. 2018. Antiherpes virus activity of extracts from Artemisia chamaemelifolia Vill. Compt. Rend. l’Acad. Bulg. Sci., Vol 72, No11, pp.1475-1483.
12. Voleva, S, V. Manolov, S. Angelova, V. Vasilev, Z. Gramatikova, I. Bogov, B. Marinov, S. Shishkov. 2018. Parvovirus B19 changes iron homeostasis during pathological pregnancy. Clin. Pract., 15 (1): 481-487
13. Chayrov R., E. Stylos, M. Chatziathanasiadou, K. Chuchkov, A. Tencheva, A. Kostagianni, T. Milkova, A. Angelova, A. Galabov, S. Shishkov, D. Todorov, A. Tzakos, I. Stankova. 2018. Tailoring acyclovir prodrugs with enhanced antiviral activity: rational design, synthesis, human plasma stability and in vitro evaluation. Amino Acids. DOI: 10.1007/s00726-018-2590-y.
14. Voleva, S., A. Pavlova, P. Genova-Kalu, V. Manolov, B. Marinov, St. Shishkov. 2017. Infectious agents during pregnancy – preliminary study. Probl. Inf. Parasit. Dis., 45 (2): 90-98.
15. Ivanova, S., P. Genova-Kalou, S. Voleva, S. Angelova, B. Marinov, S. Shishkov. 2016. Infectious Agents and Miscarriage in Bulgaria. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 25 (1): 1-10.
16. Zahmanov, G., K. Alipieva, P. Denev, D. Todorov, A. Hinkov, S. Shishkov, S. Simova, M.I. Georgiev. 2015. Flavonoid glycosides profiling in dwarf elder fruits (Sambucus ebulus L.) and evaluation of their antioxidant and anti-herpes simplex activities. Industrial Crops and Products, 63: 58–64.
17. Todorov, D., D. Pavlova, A. Hinkov, K. Shishkova, D. Dragolova, V. Kapchina-Toteva, S. Shishkov. 2015. Effect of extracts derived from Teucrium chamaedrys L. on Herpes simplex virus type 2. Compt. rend. Acad. bulg. Sci., 68 (12): 1519-1526.
18. Todorov, D., K. Shishkova, D. Dragolova, A. Hinkov, V. Kapchina-Toteva, S. Shishkov. 2015. Antiviral activity of medicinal plant Nepeta nuda. Biotechnol. Biotechnol. Equipment, 29 (Suppl. 1): 39-S43.
19. Todorov, D., A. Hinkov, K. Shishkova, S. Shishkov. 2014. Antiviral potential of Bulgarian medicinal plants. Phytochem. Rev., 13 (2): 525-538.
20. Shishkova K., I. Tsekov, R. Popov, S. Shishkov, Z. Kalvatchev. 2014. PCR Systems for Detection of Novel Elusive Human Pathogens Torque Teno Viruses (TTVs) in Bulgaria. Compt. Rend. l’Acad. Bulg. Sci., 67 (8):1175-1186.
21. Todorov, D., M. Dimitrova, K. Shishkova, J. Yordanova, V. Kapchina-Toteva, S. Shishkov. 2013. Comparative anti-herpes effects of the chloroform in vitro and in vivo extracts derived from Lamium album L. Bulg. J. Agricultural Sci., 19 (2): 190-193.
22. Kostova, K., Hinkov, A., Shishkov, S., Todorov, D., Dimitrova, M., Yordanowa, Z., Kapchina-Toteva, V. 2011. Antiherpes 1 Activities of Some Medical Plants from the Lamiaceae. Antiviral Res., 90 (2): A56.
23. Stankova, I., S. Shishkov, K. Kostova, A. Galabov. 2010. New Analogues of Acyclovir^® - Synthesis and biological actrivity. Z. Naturforsch., 65: 29-33.
24. Panteva, M. Varadinova, T., Shishkov, S. Hrisati, S., Bontchev, P. 1997. Effect of Complexes of amino acids with biometals - Influence on virus-cell interaction. Amino Acids, 13(1): 75
25. Dolashka-Angelova, P., L. Velkova, S. Shishkov, K. Kostova, A. Dolashki, I. Dimitrov, B. Atanasov, B. Devreese, W. Voelter, J. Van Beeumen. 2010. Glycan structures and antiviral effect of the structural subunit RvH2of Rapana hemocyanin. Carbohydr. Res., 345 (16): 2343-2347.
26. Velkova, L., D. Todorov, I. Dimitrov, S. Shishkov, J. Van Beeumen, P. Dolashka-Angelova. 2009. Rapana venosa hemocyanin with antiviral activity. Biotechnol. Biotechnological Equipment, 23, (Sp. Ed.): 606-610.
27. Stankova, I., K. Chuchkov, S. Shishkov, K. Kostova, L. Mukova, A. Galabov. 2009. Synthesis, antioxidative and antiviral activity of hydroxycinnamic acid amides of thiazole containing amino acid. Amino acids, 37 (2): 383-388.
28. Shishkov, S., M. Dimitrova, K. Kostova, A. Atanasova, V. Kapchina-Toteva. 2008. Micropropagation and antiviral activity of extracts from Lamium album L. Proc. Inter. Sym. "New Researches in Biotechnology", USAMV, Bucharest, Romania, Special volume, Series F, 31 - 37.
29. R Vassileva, D Taskova, Zornitsa Kamenarska& Stoyan Shishkov.2002. Comparative effect of extracts from marine organisms against replication of HSV-1 and HSV-2. Antiviral Research 53(3):A64-A64.
30. Varadinova, T., S. Shishkov, N. Ivanovska, M. Velcheva, S. Dangaaghiin, Z. Samadanghiin, Z. Yansanghiin. 1996. Antiviral and immunological activity of a new pavine alkaloid (-)-thalimonine isolated from Thalictrum simplex. Phytoth. Res., 10 (5): 414-417.
31. Shishkov, S., T. Varadinova, P. Bontchev, C. Nachev, E. Michailova. 1996. Complexes of Zinc with Picolinic and Aspartic Acids Inactivate Free Varicella-Zoster Virions. Metal-based drugs, 3 (1): 11-14.
32. Varadinova, T., P. Bontchev, C. Nachev, S. Shishkov, D. Strachilov, Z. Paskalev, A. Toutekova, M. Panteva. 1993. Mode of Action of Zn-complexes on Herpes Simplex Virus Type 1 Infection in vitro. J. Chemother., 5 (1): 3-9.
33. Pancheva, S., S. Shishkov, D. Ilieva. 1993. Effect of combined Acyclivir and Ribavirin on experimental Herpes simplex virus type 1 keratoconjunctivitis in rabbits. Acta Microbiol. Bulg., 29: 61 - 64.
34. Dimitrova, Z., B. Dimitrov, N. Manolova, S. Pancheva, D. Ilieva, S. Shishkov. 1993. Antiherpes effect of Melissa officinalis L. extracts. Acta Microbiol. Bulg., 29: 6-72.
35. Shishkov, S., S. Pancheva. 1990. Synergistic antiviral effect of acyclovir and ribavirin towards herpes simplex virus type 1 and virus of pseudorabies in vitro. Acta Microbiol. Bulg., 25: 69-75.
36. Pancheva, S., S. Shishkov. 1988. Comparative study on the activity of antiherpes substances against pseudorabies virus. Comp. Rend. l’Acad. Bulg. Sci., 41 (11): 111-114.