Home / The University / Faculties / Faculty of Biology / Structures / Departments / Cytology, Histology and Embryology / Lecturers / Assoc. Prof. Tanya Topuzova-Hristova, PhD


Director of MSc Program Cell Biology and Pathology
Room. 224
Phone: (+359 2) 8167 213
E-mail: topouzova@biofac.uni-sofia.bg


Sofia University “St Kl. Ohridski”, Faculty of Biology, Chair of Cytology Histology & Embryology

8, Dragan Tsankov Blvd., Sofia-1164, R.224

Position Assoc. Professor in Biology (Cell biology)
Main Courses Lecturer in Cell biology; Cytology, histology and embryology; General biology (for BSc); Control mechanisms of cell proliferation (for MSc)
Director of Master Program in Cell Biology and Pathology
Academic experience

1996 – 2000 – Assistant professor at the Chair of Cytology Histology & Embryology, Faculty of Biology, Sofia University

2001-2004 – Senior assistant professor at the Chair of Cytology Histology & Embryology, Faculty of Biology, Sofia University

2004-2014 - Major assistant professor at the Chair of Cytology Histology & Embryology, Faculty of Biology, Sofia University

2014 – onwards – Associate Professor at the Chair of Cytology Histology & Embryology, Faculty of Biology, Sofia University

Education & academic awards

2008 - PhD in Cell biology, Faculty of Biology, Sofia University St Kliment Ohridski,

1993 - Master in Biology with specialization Cell and developmental biology, Faculty of Biology, Sofia University St Kliment Ohridski,

Skills & competences

Organization and conduction of lectures

Organization and equipment of laboratories for research and training of undergraduates and young researchers.

Supervision of Master and PhD theses

Most relevant scientific papers


Topouzova-Hristova, T. (2008) Influence of halogenated inhalational anesthetics on integrity and reparative abilities of lung cells. PhD Thesis, Bulgarian Academy of Sciences.


  1. T. D. Andreeva, S. D. Petrova, K. Mladenova, V. Moskova-Doumanova, T. Topouzova-Hristova, Y. Petseva, N. Mladenov, K. Balashev, Z. Lalchev, J.A. Doumanov “Effects of Ca2+, Glu and GABA on hBest1 and composite hBest1/POPC surface films”, Colloids and Surfaces B: Biointerfaces, vol. 161, 2018.
  2. E. Haladjova, S. Halacheva, D. Momekova, V. Moskova-Doumanova, T. Topouzova-Hristova, K. Mladenova, J. Doumanov, M. Petrova, S. Rangelov “Polyplex Particles Based on Comb-Like Polyethylenimine/Poly(2-ethyl-2-oxazoline) Copolymers: Relating Biological Performance with Morphology and Structure”, Macromol. Biosci., 1700349, https://doi.org/10.1002/mabi.201700349, 2018.
  3. K. Mladenova, S. D. Petrova, T. D. Andreeva, V. Moskova-Doumanova, T. Topouzova-Hristova, Y. Kalvachev, K. Balashev, Sh. S. Bhattacharya, Ch. Chakarova, Z. Lalchev, J. A. Doumanov. „Effects of Ca2+ ions on bestrophin-1 surface films“, Colloids and Surfaces B: Biointerfaces 149 (1), р. 226–232, 2017.
  4. R. Kalinova, J. A. Doumanov, K. Mladenova, D. Janevska, M. Georgieva, G. Miloshev, T. Topouzova-Hristova, I. Dimitrov “Rational Design of Polypeptide-Based Block Copolymer for Nonviral Gene Delivery”, Chemistry Select, vol. 2, page 12006 – 12013, DOI: 10.1002/slct.2017024032017, 2017.
  5. E. Haladjova, M. Kyulavska, J. Doumanov, T. Topouzova-Hristova, P. Petrov “Polymeric vehicles for transport and delivery of DNA via cationic micelle template method”, Colloid Polym Sci, https://doi.org/10.1007/s00396-017-4193-7, 2017.
  6. J. Doumanov, K. Mladenova, T. Topouzova-Hristova, S. Stoitsova, S. Petrova, “Effects of vipoxin and its components on HepG2 cells”, Toxicon, Volume 94, Pages 36–44, 2015.
  7. A. Kostadinova, T. Topouzova-Hristova, A. Momchilova, R.Tzoneva and M.R. Berger. Anti-tumor lipids – structure, functions and medical application. In: “Advances in Protein Chemistry and Structural Biology 101”, Ed.: R. Donev, Elsevier Inc., Pages 27-66, 2015.
  8. E. Radoslavova Haladjova, S. S. Halacheva, V. Posheva, E. Peycheva, V. Moskova-Doumanova, T. Topouzova-Hristova, J. Doumanov, S. M. Rangelov “Comb-like Polyethyleneimine-based Polyplexes: Balancing Toxicity, Cell Internalization, and Transfection Efficiency via Polymer Chain Topology”, Langmuir 31 (36), p 10017–10025, 2015.
  9. R. Veleva, B. Petkova, V. Moskova-Doumanova, J. Doumanov, M. Dimitrova, P. Koleva, K. Mladenova, S. Petrova, Zh. Yordanova, V. Kapchina-Toteva, T. Topouzova-Hristova “Changes in the functional characteristics of tumor and normal cells after treatment with extracts of white dead-nettle”, Biotechnology & Biotechnological Equipment, Volume 29, Issue 1, pages 181-188, 2015.
  10. R. Skrobanska, A. Evangelatov, N. Stefanova, T. Topouzova-Hristova, A. Momchilova and R. Pankov, “Cell proliferation in in vivo-like three-dimensional cell culture is regulated by sequestration of ERK1/2 to lipid rafts”, Cell Proliferation, 47, 4, 336–346, 2014.
  11. M. Dimitrova, I. Ivanov, R. Todorova, N. Stefanova, V. Moskova-Doumanova, T. Topouzova-Hristova, V. Saynova, E. Stephanova, “Comparison of the activity levels and localization of dipeptidyl peptidase IV in normal and tumor human lung cells”, Tissue & cell, 44, 74-79, 2012.
  12. E. Stephanova, T. Topouzova-Hristova and R. Konakchieva “Mitochondria are involved in stress-response of A549 alveolar cells to halothane toxicity”, Toxicology in vitro 22(3):688-694, 2008.
  13. E. Stephanova, T. Topouzova-Hristova, R. Hazarosova, V. Moskova “Halothane-induced alterations in cellular structure and proliferation of A549 cells”, Tissue Cell 40 (6): 397-404, 2008.
  14. T. Topouzova-Hristova, R. Hazarosova, B. Bandreva, E. Stephanova “Halothane does not directly interact with genome DNA of A549 cells”, Folia Biologica (Praha), 53 (5), 174-180 2007.
  15. E. Stephanova, V. Valtcheva-Sarker, T. Topouzova-Hristova, Z. Lalchev “Influence of volatile anaesthetics on lung cells and lung surfactant”, Biotechnol. & Biotechnol. Eq., 21 (4), 393-398, 2007.
  16. T. Topouzova-Hristova, P. Daza, G. Herdugo, E. Stephanova “Volatile anaesthetic halothane causes DNA damage in A549 lung cells”, Toxicology in vitro, v.20 (5), 585-593, 2006.
Scientific projects during last 5 years

1. “Design of Novel Supramolecular Nanoparticles: Spherical Nucleic Acids with Polymeric and Liposomal Cores” DN 19/8, 10.12.2017/ Participant


3. “Novel Polymers, Polymeric Nanoparticles and Nanocapsules for Delivery of Biological Molecules” DFNI-TO2/7/2014/ Participant

4. “New three-dimensional in vivo-like cell culturing system as a model for fibroblast differentiation. Role of Rho family GTPases in the process” DDVU02-9, 2010/ PI

5. "Morphological-functional status and creation of an information system for the in vitro cultivated medicinal plant Lamium album L." Grant No DТК-02-29/2009/ Participant

Educational projects


BG051PO001-3.1.03-0001 / 2013 "Qualification of pedagogical specialists" Activity IV.4 "Teacher training for the formation of knowledge, skills and competences for student assessment"; BG051PO001-3.3.07-0002 / 2013 project "Student Practices"; BG051PO001-3.1.04 / 2013 "Improving the quality of general education"; BG051PO001-3.3.06 -0059/2013 Fundamental and applied training of PhD students, postdoctoral students, post-graduate students and young scientists in interdisciplinary biological fields and innovative biotechnology .;


BG05M20P001-2.002-0001 "Student Practices - Phase 1"