Софийски университет "Св. Климент Охридски"

ISOLATION AND PURIFICATION OF TYROSINASE FROM DIFFERENT PLANT SOURCES

POLINA VELICHKOVA¹, DESISLAVA MARINKOVA¹, SPASKA YANEVA²*, LYUBOV YOTOVA¹

1 – University of Chemical Technology and Metallurgy, Department of Biotechnology, Sofia, Bulgaria
2 – University of Chemical Technology and Metallurgy, Department of Bases of Chemical Technology, Sofia, Bulgaria
*Corresponding author: sp_yaneva@uctm.edu

Keywords: enzymes, tyrosinase sources, isolation, purification

Abstract: Tyrosinases from potato (Solanum tuberosum), banana (Musa acuminata) and Agaricus bisporus were isolated by two methods. The obtained filtrates were purified by precipitation with ammonium sulfate and ice-cold ethanol.
The aim of this study was to compare specific activities of enzymes, laboratory isolated from different sources using industrial purified tyrosinase as a reference.
The temperature, pH optimums and kinetic parameters were determined.

FULL TEXT