EFFECT OF AUXIN ON PROTEIN CONTENT DURING IN VITRO RHIZOGENESIS OF SYRINGA VULGARIS L.
PETYA PARVANOVA1, TEODORA LYUBOMIROVA2*, NIKOLINA TZVETKOVA2, VLADIMIR ILINKIN2
1 – Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria
2 – University of Forestry, 10 Kliment Ohridski Blvd., 1797 Sofia, Bulgaria
*Corresponding author: tlyubomirova@gmail.com
Keywords: auxin, common lilac, IBA, in vitro, pulse treatment, rooting in vitro
Abstract: Protein accumulation was studied during the in vitro rooting of axillary shoots of Syringa vulgaris L. on half-strength MS medium supplemented with 7.5 mg l-1 IBA (Indole-3-butyric acid) and on auxin free one (control). The aim of the study was to determine the most appropriate duration of auxin application by monitoring protein content as a suitable marker for the rooting of Syringa vulgaris in vitro. The levels of this biochemical parameter were monitored on 12th, 24th, 36th, 48th, 60th and the 72nd hour after the beginning of the experiment. Our results showed that the protein content in auxin-treated explants was generally higher than in the control, except on the 36th hour. The beginning of root formation was indicated by a sharp decrease in total protein accumulation on IBA containing medium. On the basis of the results the most appropriate time for a transfer of in vitro cultivated shoots from inductive onto expressive rooting medium is around the 36th hour after the beginning of the cultivation.