ANTI-HERPES EFFECTS OF IN VITRO AND IN VIVO EXTRACTS DERIVED FROM LAMIUM ALBUM L.
D. TODOROV1*, A. HINKOV1, M. DIMITROVA2, K. SHISHKOVA1, D. DRAGOLOVA2, V. KAPCHINA-TOTEVA2, S. SHISHKOV1
1 – Laboratory of Virology
2 – Department of Plant Physiology, Faculty of Biology, St. Kl. Ohridski Sofia University, 8 Dragan Tzankov Blvd, 1164 Sofia, Bulgaria
*Corresponding author: dani_todorov@abv.bg
Keywords: Antiherpes activity, Herpes Simplex Virus, Lamium album L.
Abstract: The genus Lamium (Lamiaceae) comprises of about 40 species. Lamium album L. possesses anti-inflammatory, astringent and anti-septic activity and is widely utilized in several of the treatments. In our in vitro study, water extracts received by lyophilization extraction as well as ethanol extracts obtained by thermostat extraction from in vivo and in vitro propagated plants were tested for antiviral activity.
The water extracts inhibited the replication of Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in MDBK cells significantly without apparent cytotoxicity. The 50% effective concentration (EC50) of the water in vitro extract was 1130 μg/ml for HSV-1 and 990 μg/ml for HSV-2. The EC50 of the water in vivo extract was 940 μg/ml and 1970 μg/ml, respectively. The replication of both viral types was suppressed over 95% of the in vitro extract applied in maximal nontoxic concentrations. The ethanol extracts shown no antiviral effect. The extract applied in MTC inactivated the entry of HSV-1 above 60% (Δlog0,5).