MICROPROPAGATION OF PAULOWNIA SPECIES AND HYBRIDS
ATANAS CHUNCHUKOV*, SVETLA YANCHEVA
Department of Genetics and Plant Breeding, Plant Biotechnology Laboratory,
Agricultural University Plovdiv, Bulgaria
*Corresponding author: muad@abv.bg
Keywords: micropropagation, Paulownia, MS, DKW, QL, McC, N6
Abstract: The present research shows the possibility for micropropagation of three different genotypes of Paulownia (P. elongata; P. tomentosa x P. fortunei hybrid and (P. elongata x P. tomentosa) x P. elongata complex hybrid) and some preliminary results concerning the effect of the main factors influencing the growth characteristics and propagation efficiency.
Basal media MS, DKW, QL, McC and N6 enriched with ВАР (0.5 mg/l) and IBA (0.01 mg/l) were studied for multiplication efficiency. MS basal salt composition induced higher multiplication coefficient in P. tomentosa x P. fortunei hybrid (3,9) (P. elongata x P. tomentosa) x P. elongata complex hybrid (2) and P. elongata (1,8). Additionally, the effect of cultural vessels was determined towards optimization of the propagation efficiency. The reaction of the genotypes differed generally in the mean number of shoots per explant, number of internodes and mean shoot length. The obtained plants were rooted (100%) on MS basal medium enriched with 0,1 mg/l IBA and successfully adapted ex vitro with surviving up to 96%.