MULTIFUNCTIONAL ALGOLOGY LAB FOR CO2 FIXATION BY SCENEDESMUS OBLIQUUS WITH POTENTIAL FOR SYNTHESIS OF HIGH-VALUE PRODUCTS
SNEZHANA RUSINOVA-VIDEVA1, ALEXANDAR ADAMOV1,ANNA BRACHKOVA2, MAYA MARGARITOVA ZAHARIEVA3, DIMITRINA ZHELEVA-DIMITROVA4, VESSELA BALABANOVA4, RENETA GEVRENOVA4, YANA ILIEVA3, MILA KALEVA3,TANYA KIM3, ALMIRA GEORGIEVA5,6, MILKA MILEVA5,HRISTO NAJDENSKI3, ALEXANDER KROUMOV2*
1 Department of Biotechnology, Laboratory cellular biosystems, The Stephan Angeloff Institute of Microbiology, BAS, 4000 Plovdiv, Bulgaria
2Department of Biotechnology, Laboratory of bio-conversion and biosynthesis of microbial metabolites, Sofia 1113, Bulgaria,
3Department of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology, BAS,Sofia 1113, Bulgaria
4Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Medical University – Sofia, 2 Bulgaria
5 Department of Virology, The Stephan Angeloff Institute of Microbiology, BAS, Bulgaria
6Institute of Neurobiology, BAS,Sofia, Bulgaria
* Corresponding author: adkrumov@gmail.com
Keywords: photobioreactor, Scenedesmus obliquus, engineering, antimicrobial activity
Abstract: Several different types of photobioreactors (PBRs) were designed in order to improve the selection of microalgal strains of freshwater algae isolated from Bulgarian locations. The criteria for strain selection are based on their potential for a high specific growth rate and proven ability to synthesize biologically active metabolites with antimicrobial activity. Experimental sets of batch processes were performed with the promising Scenedesmus obliquus 8610 strain cordially provided by National Algae Culture Collection-Plovdiv (PACC). The preliminary experiments and results on the antimicrobial activity of the extracts from S. obliquus biomass were promising. By calculating the nutrients of the medium using a linear programming procedure and by applying the M-8 modified medium recipe, we were able to achieve maximum biomass concentration in the flat plate-PBRs under given working conditions. The strategy to find new valuable metabolites from this strain passed through the manipulation of various state parameters and stress factors. We believe that by completing this research, we will be able to offer a robust strain and a technological protocol for its subsequent industrial application.