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Head of the group

Assist. Prof. Dr. Nikolay Grozev



Group members

Prof. DSc. Konstantin Balashev


Assist. Prof. Dr. Kristina Mircheva


Assist. Prof. Dr. Petar Georgiev


Dr Silvia Simeonova


Prof. DSc Ivan Panaiotov (Emeritus)


Prof. DSc Christian Vassilieff (Emeritus)


Prof. Dr. Tzvetanka Ivanova (Emeritus)



Research interests

Study of the state, electrical and rheological properties of the model 2D-systems: monolayers, liposomial bilayers and suspensions. Interfacial and temporal organization of the lipolysis. Mechanisms of degradation of the polyesters and lipid micro- and nano-capsules. Photochemical reactions at the interface. State, electrical and rheological properties of the biopolymer model monolayers from the plant kingdom. Atomic Force Microscopy (AFM).


International cooperation

Laboratory of Enzymology at Interfaces and Physiology of Lipolysis, CNRS, Marseilles, France

Laboratory of Particle Vectorisation, University of Angers, France

Laboratory of Enzyme Design, Novo Nordisk, Denmark

Nanoscience Center, University of Copenhagen, Denmark


Teaching activity

The Laboratory is engaged in the education of students from all academic levels

B. Sc. courses

Lectures and practical exercises in: physical chemistry and colloidal chemistry

M. Sc. courses

Master program: Biophysical Chemistry in Medicine and Pharmacology

Lectures and practical exercises in: Biophysical Chemistry, Colloidal dispersions in medicine and pharmacy

Master Thesis

PhD Thesis


Selected papers

I. Panaiotov, R. Verger, “Enzymatic reactions at the interface”, in Physical Chemistry of Biological Interfacers”

ed. by W. Norde and A. Baszkin, Elzevier (2000), 359.


C.Vassilieff, I.Panaiotov, E. D. Manev, J.E. Proust and Tz. Ivanova, “Kinetics of liposome disintegration from foam film studies: Effect of the lipid bilayer phase state” Biophysical Chemistry: 58 (1996) 97.


N.Grozev, V..Aguié-Béghin, Tz.Ivanova, S.Baumberger, B.Cathala, R.Douillard, I.Panaiotov,“State, electrical and rheological properties of model and dioxan isolated lignin films at the air-water interface” Coll. Polym. Sci., 280 (2002) 798.


I. Minkov, Tz. Ivanova, I. Panaiotov, J. Proust, P. Saulnier, “Reorganisation of lipid nanocapsules at air-water interface: 1. Kinetics of surface film formation” Colloids and Surfaces B: Biointerfaces, 45 (2004) 32.


K. Mircheva, I. Minkov, Tz. Ivanova, I. Panaiotov, J.E.Proust, R. Verger, “Comparative study of lipolysis by PLA2 of DOPC substrates organized as monolayers, bilayer vesicles and nanocapsules”,Colloids and Surfaces B:Biointerfaces, 67 (2008) 107.


K. Mircheva, Tz. Ivanova, I. Panaiotov, V. Dusel, F. Boury, “Enzymatic proteolysis of alfa gliadin monolayer spread at the air water interface”, J. Coll.Int. Sci., 347 (2010) 69.


K. Mircheva, Tz. Ivanova, I. Panaiotov, R. Verger, ”Hydrolysis of mixed monomolecular films of tricaprylin/dilauroylphosphatidylcholine by lipase and phospholipase A2”, Colloids and Surfaces B: Biointerfaces , 86 (2011) 71.


K. Balashev, Tz.Ivanova, K. Mircheva, I.Panaiotov, „Savinase proteolysis of insulin Langmuir monolayers studied by surface pressure and surface potential measurements accompanied by atomic force microscopy (AFM) imaging” Journal of Colloid and Interface Science 360 (2) (2011) 654.


I. Minkov, K. Mircheva, N.Grozev, Tz. Ivanova, I. Panaiotov – Properties of mixed monolayers of clinical lung surfactant, serum albumin and hydrophilic polymers, Colloids and Surfaces B: Biointerfaces, 101 (2013) 135-142.


P. Georgiev, A. Bojinova, B. Kostova, D. Momekova, T. Bjornholm, K.Balashev, „Implementing atomic force microscopy (AFM) for studying kinetics of gold nano-particle's growth” Colloids Surf A: Physicochem Eng Aspects, 434 (2013) 154


K. Mircheva, M. Gonnet, K. Balashev, Tz. Ivanova, F. Boury, I. Panaiotov, “Properties of -carotene and retinoic acid in mixed monolayers with dipalmitoylphosphatidylcholine (DPPC) and Solutol”,Colloids and Surfaces A: Physicochem Eng Aspects, .460 (2014) 209.


Tz. Ivanova, K. Mircheva, K. Balashev, I. Minkov, P. Saulnier and I. Panaiotov, “Interfacial behavior of lipid nanocapsules spread on model membrane monolayers”, Coll. Polym. Sci, 292 (6) (2014) 1307.


A.C.Groo, K. Mircheva, J. Bejaud, C. Aihas, I.Panaiotov, P. Saulnier, Tz. Ivanova, F. Lagarce, - Development of 2D and 3D Mucus Models and Their Interactions with Mucus Penetrating Paclitaxel- loaded Lipid Nanocapsules, Pharmaceutical Research, 31 (7) (2014) 1753.