Home / The University / Faculties / Faculty of Biology / Structures / Departments / Biophysics and radiobiology / Lecturers / Prof. Vasiliy Goltsev, PhD - guest lecturer

   

Phone: +359 2 8167 370,
E-mail: goltsev.v@uni-sofia.bg

Prof. Vasilij Goltsev,
Dept. Biophysics and Radiobiology, Faculty of Biology, St. Kl. Ohridski University of Sofia,
Dragan Tzankov Blvd. 8, 1164, Sofia Bulgaria

 

Major Fields of Scientific Research (Keywords):

Biophysics; Photobiophysics; Biophysics of Photosynthesis; Stress and adaptation; Chlorophyll a Fluorescence, Delayed Fluorescence, Mathematical Modeling of Biological Systems; Artificial Neural Networks; Nonlinear Thermodynamcs; Biomembranes

Brief notes:

Vasilij Goltsev was born at 1950 in Russia. He graduated MS (1967 – 1972) and PhD (1976 – 1979) at the Moscow State University, Faculty of Biology, Department of Biophysics. He has been a lecturer in the Dept. Biophysics and Radiobiology in Sofia University since 1980 and he was the head of this department for the periods 1991-2004 and 2008-2012. Head of Laboratory of Photobiophysics. Expert in biophysics of photosynthesis, fluorescent methods for analysis of the photosynthesis, stress reactions in plants, mathematical modelling of biological processes.

Educational activity:

Courses of lectures and exercises:

  • “Biophysics and Radiobiology” for BSc Programs “Molecular Biology” and “Biotechnology”;
  • “Photobiology” for BSc Program “Molecular Biology”;
  • “Mathematical Modelling of Biological processes” for BSc Program “Molecular Biology”;
  • “Mathematical Modelling of Biophysical processes” for MSc Program “Biophysics”;
  • “Quantum- and Photobiophysics” for MSc Program “Biophysics”;
  • “Biophysics of Photosynthesis” for MSc Program “Biophysics”

Major Fields of Scientific Research:

At last years in the Lab Photobiophysics a biophysical approach for multi-parametric estimation of the physiological state and stress reaction in plants at in vivo and in situ conditions has been developed. The approach is based on measuring and analysis of luminescent and optical signals emitted from the leaf surface. The most efficient way to receive information from plant living tissues about photosynthetic reactions is an analysis of light induced transients of prompt and delayed chlorophyll fluorescence (see the Figure). They provide information about quantum efficiency and electron transport rate in different sites of the photosynthetic electron transport chain, as well as about the collection and redistribution of light energy in the photosystem II antenna complexes. For prompt fluorescence signal analysis the approach known as JIP-test was used. To link data from induction curves of prompt and delayed chlorophyll a fluorescence and of 820 nm light reflection with general physiological characteristics of plants an approach was developed. It is based on the artificial neural networks, which are capable to recognize the taxonomic classification of the plant or the level of water stress in the leaves with high level of confidence.

Goltsev - 2013 - Fig. 01 - Eng

 

Since the method is non-invasive and highly informative, it has become a powerful tool for photosynthetic researches at in vivo conditions and it is perspective for environmental monitoring of plant ecosystems. It allows to link genotype (taxonomical and functional) characteristics of the plant samples to their phenotype, expressed by the values of a group of experimental parameters as a kind of “barcode” that uniquely represent the functional state of the investigated plant species.

Scientific cooperation with Bulgarian and foreign scientific organizations:

Bulgarian collaborators:

Institute of Plant Physiology and Genetics, BAS, Sofia, Bulgaria;
Agriculture University, Plovdiv, Bulgaria;
AgroBioInstitute, Sofia, Bulgaria

 

Foreign collaborators:

Bioenergetics Laboratory, University of Geneva, Geneva, Switzerland;
Department of Plant Physiology, Warsaw University of Life Sciences SGGW, Warsaw, Poland;
Freie Universität Berlin, Berlin, Germany;
Department of Environmental Biology, Sapienza University of Rome, Rome, Italy;
Department of Agri-Food Production and Environmental Science (DISPAA), Section of Soil and Plant Science, University of Florence, Piazzale delle Cascine, Firenze, Italy;
Department of Chemistry and Biochemistry, University of Quebec in Montreal, Montreal, Quebec, Canada;
Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia;
Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, Russia;
Moscow State University, M.V. Lomonosov, Faculty of Biology, Department Biophysics, Moscow, Russia
Department of Biochemistry, Department of Plant Biology, and Center of Biophysics & Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA;
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China;
Shandong Key Laboratory of Crop Biology, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong, China

Principal Publications:

  1. Abdallah Oukarroum, Filippo Bussotti, Vasilij Goltsev, Hazem M. Kalaji (2015) Correlation between reactive oxygen species production and photochemistry of photosystems I and II in Lemna gibba L. plants under salt stress. Environmental and Experimental Botany, 109: 80 - 88
  2. Elisabetta Salvatori; Lina Fusaro; Elena Gottardini; Martina Pollastrini; Vasilij Goltsev; Reto J Strasser; Filippo Bussotti (2014) Plant stress analysis: application of Prompt, Delayed Chlorophyll Fluorescence and 820 nm Modulated Reflectance. Insights from independent experiments, Plant Physiology and Biochemistry, 85, 105-113, doi: 10.1016/j.plaphy.2014.11.002
  3. Goltsev V. N., Kalaji M. H., Kouzmanova M. A., Allakhverdiev S. I. (2014) Variable and Delayed Chlorophyll a Fluorescence – Basics and Application in Plant Sciences. – Moscow–Izshevsk: Institute of Computer Sciences, 220 pp.
  4. Izabela A. Samborska, Vladimir Alexandrov, Leszek Sieczko, Bożena Kornatowska, Vasilij Goltsev, Magdalena D. Cetner, and Hazem M. Kalaji (2014) Artificial neural networks and their application in biological and agricultural research. NanoPhotoBioSciences, 2, 14-30
  5. Vladimir Aleksandrov, Vasilena Krasteva, Momchil Paunov, Maria Chepisheva, Margarita Kousmanova, Hazem M. Kalaji and Vasilij Goltsev (2014) Deficiency of some nutrient elements in bean and maize plants analyzed by luminescent method. Bulgarian Journal of Agricultural Science, 20 (Supplement 1) 2014, 24–30
  6. Hazem Kalaji, Abdallah Oukarroum, Vladimir Alexandrov, Margarita Kouzmanova, Marian Brestic, Marek Zivcak, Izabela A. Samborska, Magdalena D. Cetner, Suleyman I. Allakhverdiev,Vasilij Goltsev (2014) Identification of nutrient deficiency in Maize and Tomato plants by in vivo chlorophyll a fluorescence measurements. Plant Physiology and Biochemistry, 81, 16 – 25. http://dx.doi.org/10.1016/j.plaphy.2014.03.029
  7. Hazem M. Kalaji, Anjana Jajoo, Abdallah Oukarroum, Marian Brestic, Marek Zivcak, Izabela Samborska, Magdalena D. Cetner, Vasilij Goltsev, Richard J. Ladle, Piotr Dąbrowski, Parvaiz Ahmad (2014) The use of chlorophyll fluorescence kinetics analysis to study the performance of photosynthetic machinery in plants. In: P. Ahmad (Ed): Emerging Technologies and Management of Crop Stress Tolerance. Elsevier, Vol. II, 347 – 384. DOI: http://dx.doi.org/10.1016/B978-0-12-800875-1.00015-6
  8. Hazem Kalaji, …V. Goltsev et al. Frequently Asked Questions about chlorophyll fluorescence: practical and technical issues (2014) Photosynthetic Research, 122:121–158, DOI 10.1007/s11120-014-0024-6
  9. Hazem M.Kalaji, Vasilij Goltsev, Marián Brestič, Karolina Bosa, Suleyman I. Allakhverdiev, Reto J. Strasser, and Govindjee (2014) In vivo Measurements of Light Emission in Plants In: Photosynthesis: Open Questions and What We Know Today, Allakhverdiev S. I., Rubin A. B., Shuvalov V.A. (eds.). Institute of Computer Science, Izhevsk–Moscow, pp. 1-40
  10. YANG Cheng, LI Peng-Min, ZHANG Zi-Shan, Vasilij Goltsev, GAO Hui-Yuan (2013) Arising of Chlorophyll Delayed Fluorescence and Its Application in Photosynthesis Research. Zhiwu Shengli Xuebao/Plant Physiology Journal 2013, 49 (12): 1277-1285 (in Chinese)
  11. Gao J, Li P, Ma F, Goltsev V (2014) Photosynthetic performance during leaf expansion in Malus micromalus probed by chlorophyll a fluorescence and modulated 820 nm reflection. Journal of Photochemistry and Photobiology B: Biology, 137:144–150. doi:http://dx.doi.org/10.1016/j.jphotobiol.2013.12.005
  12. Krasteva Vassilena, Alexandrov Vladimir, Chepisheva Mariya, Dambov Shterjan, Stefanov Detelin, Yordanov Ivan, Goltsev Vasilij (2013) Drought induced damages of photosynthesis in bean and plantain plants analyzed in vivo by chlorophyll a fluorescence. Bulgarian Journal of Agricultural Science, 19 (2), 39–44
  13. Oukarroum A, Goltsev V, Strasser RJ (2013) Temperature Effects on Pea Plants Probed by Simultaneous Measurements of the Kinetics of Prompt Fluorescence, Delayed Fluorescence and Modulated 820 nm Reflection. PLoS ONE 8(3): e59433. doi:10.1371/journal.pone.0059433
  14. Plyusnina T. Yu., E. N. Voronova, V. N. Goltsev, S. I. Pogosyan, O. V. Yakovleva, G. Yu. Riznichenko, A. B. Rubin Reduced model of photosystem II and its use to evaluate the photosynthetic apparatus characteristics according to the fluorescence induction curves, Computer Research and Modeling, 2012, vol. 4, no. 3, pp. 943–958 (Russian).
  15. Kalaji Hazem M., Govindjee, Vasilij Goltsev, Karolina Bosa, Suleyman I. Allakhverdiev, Reto J. Strasser (2012) Experimental in vivo measurements of light emission in plants: A perspective dedicated to David Walker, Photosynthesis Research 114:69–96
  16. Vasilij Goltsev, Ivelina Zaharieva, Petko Chernev, Margarita Kouzmanova, Hazem M. Kalaji, Ivan Yordanov, Vasilena Krasteva, Vladimir Alexandrov, Detelin Stefanov, Suleyman I. Allakhverdiev, Reto J. Strasser (2012) Drought-induced modifications of photosynthetic electron transport in intact leaves: Analysis and use of neural networks as a tool for a rapid non-invasive estimation. Biochim. Biophys. Acta (2012), 1817: 1490-1498.
  17. Stanislav Zapryanov, Vasilii Goltsev, Bojidar Galutsov, Metody Gelev, Alexander Blagoev (2012) Influence of the soft X-ray plasma focus radiation on live microorganisms, EPJ Applied Physics, 58, 11201 doi:10.1051/epjap/2012110322 Zapryanov, S., Goltsev, V., Galutsov, B., Gelev, M., & Blagoev, A. (2012). Influence of the soft X-ray plasma focus radiation on live microorganisms. The European Physical Journal Applied Physics, 58(01).
  18. Ivan Yordanov, Detelin Stefanov, Vasilena Krasteva, Maria Gourmanova, Vasilij Goltsev (2012) Drought stress responses in plants – molecular biology, physiology and agronomical aspects. Agricultural Sciences, IV(8): 7-20
  19. Goltsev, V., Gurmanova, M., Kouzmanova, M., Yordanov, I., Qiang, S., Pentland, A., Wilson, N., Chen, S., Zaharieva, I., Strasser, R.J., 2013. Analysis of Dark Drops, Dark-Induced Changes in Chlorophyll Fluorescence during the Recording of the OJIP Transient. In: Kuang T, Lu C, Zhang L (eds) Photosynthesis Research for Food, Fuel and the Future. Springer-Verlag, Berlin Heidelberg, pp 179-183.
  20. Reto J. Strasser; Merope Tsimilli-Michael; Sheng Qiang; Vasilij Goltsev (2010) Simultaneous in vivo recording of prompt and delayed fluorescence and 820 nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. BBA, 1797, 1313–1326, doi:10.1016/j.bbabio.2010.03.008
  21. Goltsev V., I. Zaharieva, P. Chernev and R. Strasser (2009) Delayed fluorescence in photosynthesis. Photosynth Res, 101, 217-232. DOI 10.1007/s11120-009-9451-1
  22. Goltsev V., I. Zaharieva, P. Chernev and R. Strasser (2009) Delayed chlorophyll fluorescence as a monitor for physiological state of photosynthetic apparatus. Biotechnology & Biotechnological Equipment, 23 (Special Edition) 452-457
  23. Gurmanova M., P. Chernev, I. Zaharieva and V. Goltsev (2009) Kinetic model of electron-transport reactions in thylakoid membranes determining chlorophyll fluorescence transients. Biotechnology & Biotechnological Equipment, 23 (Special Edition) 621-626
  24. Kirova M., G. Ceppi, P. Chernev, V. Goltsev, R.J. Strasser (2009) Using artificial neural networks for plant taxonomic determination based on chlorophyll fluorescence iduction curves. Biotechnology & Biotechnological Equipment, 23 (Special Edition) 941 - 945
  25. Yordanov I., Goltsev V., Stefanov D., Chernev P., Zaharieva I, Kirova M., Gecheva V., Strasser R.J. (2008) Preservation of PS II Electron Transport from Senescence-Induced Inactivation in Primary Leaves after Decapitation and Defoliation of Bean Plants, J. Plant Physiol. 165 (18) 1954-1963
  26. Yordanov I., Goltsev V., P.Chernev, Zaharieva I. , Stefanov D., and Strasser R. (2008) Modulated sink-source interactions preserve the PSII electron transport from senescence-induced inactivation in model system with expanded life span induced by decapitation of bean plants. In: J. Allen, E. Gantt, J.Golbeck, B. Osmond (eds). Energy from the Sun. Springer, Dordrecht, pp. 675–680
  27. Goltsev V., Tsimilli-Michael M., Chernev P., Zaharieva I., Kouzmanova M., Strasser R.J. (2008) Electromagnetic frequency spectra of samples placed in a coil that senses the electromagnetic background field: Application for leaves, chloroplasts and molecules useful in photosynthesis. In.: J.F. Allen, E. Gantt, J.H. Golbeck & B. Osmond (eds.), Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis. Springer, Dordrecht, pp. 591–596
  28. Christov, I., Stefanov, D., Velinov, T., Goltsev, V., Georgieva, K., Abracheva, P., Genova, Y., Christov, N. (2007). The symptomless leaf infection with grapevine leafroll associated virus 3 in grown in vitro plants as a simple model system for investigation of viral effects on photosynthesis. J. Plant Physiol. 164(9) 1124-1133
  29. Chernev P., V.Goltsev, I.Zaharieva, R.J.Strasser (2006). A highly restricted model approach quantifying structural and functional parameters of Photosystem II probed by the chlorophyll a fluorescence rise. Ecological Engereeng and Environtment protection, 5(2), 19-29.
  30. Kocheva K.V., Busheva M.C., Georgiev G.I., Lambrev P.H., Goltsev, V.N. (2005) Influence of short-term osmotic stress on the photosynthetic activity of barley seedlings’ Biologia Plantarum 49 (1): 145-148
  31. Goltsev V., P.Chernev, I.Zaharieva, P.Lambrev, R.J.Strasser (2005). Kinetics of delayed chlorophyll a fluorescence registered in milliseconds time range. Photosynth. Res. 84: 209-215
  32. Goltsev, V., Zaharieva, I., Chernev, P., Lambrev, P. and R. J. Strasser (2005) Origin of kinetic components of millisecond delayed chlorophyll fluorescence. In: van der Est, A. and Bruce, D. (eds.), Photosynthesis: Fundamental Aspects to Global Perspectives. Proceedings of XIII International Congress on Photosynthesis. 29 August – 3 September 2004, Montreal, Canada. International Society of Photosynthesis, pp. 211-213.
  33. Goltsev, V., Zaharieva, I., Chernev, P., Lambrev, P. and R. J. Strasser (2005). Origin of kinetic components of millisecond delayed chlorophyll fluorescence. In: van der Est, A. and Bruce, D. (eds.), Photosynthesis: Fundamental Aspects to Global Perspectives. International Society of Photosynthesis, pp. 211-213.
  34. Kapchina V., G. MIlanov, A. Zankov, D. Stefanov, S. Slavov, V. Goltsev, R. Batchvarova (2004). The changes in some photosynthestic characteristics of transgenic tobacco plants, resistant to bacteria Pseudomonas syringae pv. tabaci. Biotechnology and Biotechnological Equipments, 18(3) 74-84. http://dx.doi.org/10.1080/13102818.2004.10817124
  35. Parvanova D., A. Popova, I. Zaharieva, P. Lambrev, T. Konstantinova, S.Tanеva, A. Atanassov, V. Goltsev, D. Djilianov (2004). Low temperature tolerance of tobacco plants transformed to accumulate proline, fructans, or glycine betaine. Variable chlorophyll fluorescence evidence. Photosynthetica, 42 (2), 179-185
  36. Zaharieva I., V. Goltsev, P. Chernev, R. Strasser (2004). Temperature effects on parameters of millisecond delayed chlorophyll fluorescence – A mathematical model. In: Zrimec, M., Zrimec, A., Drobne, D. and Milani, M. (eds.), Weak Photon Emission from Living Tissues: Delayed Luminescence – Facts & Perspectives, pp. 24-28, Institute of Physical Biology, Grosuplje, Slovenia.
  37. Kocheva K., G.Georgiev, P.Lambrev, V.Goltsev, M.Karabaliev (2004). Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress. Bioelectrochemistry, 63, 121-124
  38. Lambrev P., S. Ivanov, V. Goltsev (2003). Effects of prolonged action of sub-herbicide concentrations of atrazine on the photosynthetic function of pea plants. CR Acad. Bulg. Sci., 56(3), 59-62
  39. Zaharieva I., V. Goltsev (2003). Advances on Photosystem II investigation by measurement of delayed chlorophyll fluorescence by a phosphoroscopic method. Photochem. Photobiol. 77(3): 292-298
  40. Goltsev V., I. Zaharieva, P. Lambrev, I. Yordanov, R. Strasser (2003). Simultaneous analysis of prompt and delayed chlorophyll a fluorescence in leaves during the induction period of dark to light adaptation. J. Theor. Biol. 225(2), 171-183
  41. Yordanov I., K. Georgieva, V.Velikova, Ts.Tsonev, V.Goltsev, P.Ivanov, N.Tsenov, T.Petrova (2001/2002). Responses of photosynthetic apparatus of different wheat (Triticum aestivum L.) genotypes to drought under field conditions. II. Changes in O2 evolution and chlorophyll fluorescence parameters – a possible basis for screening of drought tolerance. Genetics and Breeding, 31 (3-4), 21-30.
    PS2001 Proceedings: 12th International Congress on Photosynthesis. CSIRO Publishing: Melbourne, Australia, 2001. Available at http://www.publish.csiro.au/ps2001 (posted Dec 2001)
  42. Goltsev V., I. Yordanov, D. Stefanov, I. Zahareiva, P. Lambrev, R. Strasser (2001). Simultaneous analysis of variable and delayed chlorophyll fluorescence during induction period in photosynthetic apparatus. Proceedings of 12th International Congress on Photosynthesis, August 18-23, Brisbane, Australia. http://www.publish.csiro.au/ps2001
  43. Zaharieva I., St. Taneva, V. Goltsev (2001). effect of temperature on the luminescent characteristics in leaves of arabidopsis mutants with decreased unsaturation of the membrane lipids. Bulg. J. Plant Physiol. 27(3-4), 3-18
  44. Lambrev P., V. Goltsev (2001). pH dependence of the effects of diuron, atrazine and dinoseb on the luminescent properties of thylakoid membranes. Bulg. J. Plant Physiol. 27 (1-2), 80-95
  45. Khristov I.K., D. Stefanov, V.N. Goltsev, and P. Abrasheva (2001). Effects of Grapevine Fanleaf and Stem Pitting Viruses on the Photosynthetic Activity of Grapevine Plants Grown in vitro. Russian Journal of Plant Physiology, Vol. 48, No. 4, 2001, pp. 473–477. Translated from Fiziologiya Rastenii, Vol. 48, No. 4, 2001, pp. 551–555.
  46. Goltsev V., T. Genkov, M.Lexa and I. Ivanova (2001). Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro. Scientia Horticulturae, 89, 41-55
  47. Lambrev P., V. Goltsev (1999). Temperature affects herbicide-sensitivity of pea plants. Bulg. J. Plant Physiol. 25(3-4), 54-66
  48. Zaharieva I., St. Taneva, V. Goltsev (1999). Effect of PS II antennae size on the induction kinetics of prompt and delayed chlorophyll fluorescence. Bulg. J. Plant Physiol. 25(3-4), 17-30. Download site: http://www.bio21.bas.bg/ipp/gapbfiles/v-25/99_3-4_17-30.pdf
  49. Traikov, L., V. Goltsev and M. Markov (1999). Temperature and millimeter waves combined action on luminescence characteristics of thylakoid membranes. In: F. Bersani (ed.) “Electricity and Magnetism in Biology and Medicine” – Proceedings of Second World Congress for Electricity and Magnetism in Biology and Medicine, 1999, pp.371-373. Kluwer Academic/Plenum Publishers.
  50. Mladenova, Y., Maini, C.Mallegni, P., Goltsev, V., Vladova, R., Vinarova, K. and Rocheva, S. (1998). Siapton - An amino-acid-based biostimulant reducing osmostress metabolic changes in maize. Agro-food-Industry hi-tech, 9(6), 18-22
  51. Yordanov, I., K. Georgieva, T. Tsonev, V. Goltsev, M.Merakchiiska (1998). Effect of carbamide cytokinin 4PU30 on the photosynthesis of bean plants endured drought and high temperature stresses. In: G.Garab (ed.), Photosynthesis: Mechanisms and Effects, Vol. IV, pp. 2577-2580. Kluwer Academic Publishers. Printed in the Netherlands
  52. Goltsev V., L.Traikov, V.Hristov (1998). Effects of exogenous electron acceptors on kinetic characteristics of prompt and delayed fluorescence in atrazine inhibited thylakoid membranes. In: G.Garab (ed.), Photosynthesis: Mechanisms and Effects, Vol. V, pp. 3885-3888. Kluwer Academic Publishers. Printed in the Netherlands
  53. Zaharieva I., M. Velitchkova and V. Goltsev (1998). Effect of cholesterol and benzyl alcohol on prompt and delayed chlorophyll fluorescence in thylakoid membranes. In: G.Garab (ed.), Photosynthesis: Mechanisms and Effects, Vol. III, pp. 1827-1830. Kluwer Academic Publishers. Printed in the Netherlands
  54. Velikova, V. B., I. T. Yordanov, K. M. Georgieva, T. D. Tsonev and V. Goltsev (1998) Effects of Exogenous Polyamines Applied Separately and in Combination with Simulated Acid Rain on Functional Activity of Photosynthetic Apparatus. J. Plant Physiol. 153, 299-307
  55. Milanov G., Stefanov D., Goltsev V., Batchvarova R. (1997) Changes in the submilli- and millisecond kinetics of dark relaxation of delayed fluorescence in tobacco leaves under conditions of bacterial infection by Pseudomonas syringae pv. Tabaci. Bulg. J. Plant Physiol. 23: 35-42
  56. Yordanov I., Ts.Tsonev, V.Goltsev, M.Merakchiïska-Nikolova, K.Georgieva (1997). Gas exchange and chlorophyll fluorescence during water and high temperature stress and recovery. Probable protective effect of carbamide cytokinin 4-PU30, Photosynthetica, 33(3-4), 423-431.
  57. Yordanov I., T.Tsonev, V.Goltsev, L. Kruleva, V. Velikova (1997). Interactive effect of water deficit and high temperature on photosynthesis of sunflower and maize plants 1.Changes in parameters of chlorophyll fluorescence induction kinetics and fluorescence quenching. Photosynthetica 33(3-4):391-402.
  58. Goltsev V., Yordanov I. (1997). Mathematical model of prompt and delayed chlorophyll fluorescence induction kinetics. Photosynthetica, 33(3-4):571-586.
  59. Yordanov I., V.Goltsev, Ts.Tsonev, L.Kruleva (1995). Thermal acclimation of the photosynthetic apparatus depending on temperature and duration of treatment, Bulg. J. Plant Physiol., 21(1), 12-28.
  60. Goltsev V., I.Yordanov, Ts.Tsonev (1994). Evaluation of relative contribution of initial and variable chlorophyll fluorescence measured at different temperatures, Photosynthetica, 30(4), 629-643.
  61. Georgieva, K., I. Yordanov, V. Goltsev (1992). Temperature sensitivity of chloroplast and leaf fluorescence of acclimated and non-acclimated plants of two pea cultivars. Comp. Rend. Bulg. Acad. Sci 45, 115-117.
  62. Yordanov,I.,T., V.Goltsev, L.Kruleva (1990). Protective effect of putrescine and spermidine on the thylakoid membranes activity after high temperature treatment. In: Current Research in Photosynthesis, M.Baltscheffski (ed.), Vol. IV, 699-703. Kluwer Academic Publishers.
  63. Goltsev V., V.Zaimov (1990). Computer analysis of induction curves of millisecond delayed fluorescence. In: Proceedings of Advanced Course "Trends in Photosynthesis Research", Palma de Mallorca (Spain), 57-59.
  64. Yordanov I.T., V.Goltsev, V.Doltchinkova, L.Kruleva (1989). Effect of some polyamines on the functional activity of thylakoid membranes. Photosynthetica, 23, 314-323
  65. Georgieva K., V.Goltsev, I.Yordanov (1989). Influence of high temperature on electrophoretic mobility of thylakoid membranes from two pea cultivars. Photosynthetica, 23, 399-402
  66. V.Goltsev, P.Venedictov, V.Shinkarev (1988). Mathematical model of the millisecond delayed fluorescence. In: Techniques and New Developments in Photosynthesis Research (J.Barber and R.Malkin, eds.), NATO ASI Series, Series A,LIfe Sciences, vol. 168, 1988, pp. 281-284.
  67. Goltsev,V., O.Popov, V.Doltchinkova, I.Yordanov, V.Kagan (1988). Effects of alpha-tocopherol derivatives on photosynthetic activity of thylakoid membranes. In: Electromagnetic fields and biomembranes, (M.Markov and M.Blank, eds.), Plenum Press, New York, pp. 249-253
  68. Goltsev,V.N., Y.I.Mladenova (1987). Influence of water and salt stresses on delayed fluorescence induction kinetics in leaves of two Zea mays hybrids. Arch. Intern. Physiol. Biochim., 95, 11
  69. Goltsev,V. I.Yordanov, V.Doltchinkova, M.Kantcheva (1987). High-temperature damage and acclimation of the photosynthetic apparatus. 3. Modification of the thylakoid membrane electrophoretic mobility. Photosynthetica, 21, 189-192
  70. Yordanov I.T., V.N.Goltsev (1987). Regulation of photosynthesis at the level of phosphorylation of the light-harvesting complex and photophosphorylation. Photosynthetica, 21, 236-250
  71. Goltsev,V., I.Yordanov, T.Stoyanova, O.Popov (1987). High temperature damage and acclimation of the photosynthetic apparatus. II. The effect of mono- and divalent cations and pH on the temperature sensitivity of some functional characteristics of chloroplasts isolated from heat-acclimated and non-acclimated bean plants. Planta, 170, 478-488
  72. Yordanov I., V.Goltsev, T.Stoyanova, P.Venediktov (1987). High temperature damage and acclimation of the photosynthetic apparatus. I. Temperature sensitivity of some photosynthetic parameters of chloroplasts isolated from acclimated and non-acclimated bean leaves. Planta, 170, 471-477.
  73. Yordanov I., S.Dilova, R.Petkova, T.Pangelova, V.Goltsev, K.-H.Zuss (1986). Mechanisms of the temperature damage and acclimation of the photosynthetic apparatus. Photobiochem. Photobiophys., 12, 147-155
  74. Doltchinkova,V., T.Stoyanova, I.Yordanov, V.Goltsev (1986). Photo-induced changes of the thylakoid membrane light-scattering. II. Influence of temperature treatment. Photobiochem. photobiophys., 12, 79-82
  75. Goltsev,V., V.Doltchinkova, I.Yordanov (1986). Photo-induced changes of the thylakoid membrane light-scattering. I. Effect of the surface charge. Photobiochem. photobiophys, 12, 73-77
  76. Goltsev,V., M.Kantcheva, V.Doltchinkova, D.Kovatchev (1985). Phytohemagglutinin-induced changes of electrophoretic mobility and functional activity of pea chloroplasts. In: Cell Electrophoresis (W.Schutt, H.Klinkmann eds.), Watler de Gruyter & Co., Berlin, New York, 691-696
  77. Kantcheva,M., V.Goltsev, S.I.Todorov (1985). The electric charge of photosynthetic membranes. In: Cell Electrophoresis (W.Schutt, H.Klinkmann eds.), Watler de Gruyter & Co., Berlin, New York, 173-182
  78. Mureramanzi,S., V.Goltsev, G.Todorov, A.Zhivkov, S.Stoilov (1984). An electrooptical study of the dimentions and electric polarizability of chloroplasts and sub-chloroplast fragments. Bioelectrochem. Bioenerg., 13, 453-457
  79. Goltsev,V., M.Kantcheva, S.I.Todorov (1984). Electrical charge on chloroplast membrane surface. Part I. pH dependence of temperature inactivation of the photosynthetic membranes with different electrical charges. Bioelectrochem. Bioenerg., 13, 117-125
  80. Goltsev,V., T.Ortoidze, Z.Sokolov, D.Matorin, P.Venediktov - Delayed luminescence yield kinetics in flash illuminated green plants. Plant Sci. Lett. 19, 1980, 339-346
  81. Goltsev,V.N., P.S.Venediktov, D.A.Janumov (1980). Temperature dependence of the delayed fluorescence from wheat leaves treated with DCMU. Biocim.Biophys.Acta, 593, 133-135
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