Начало / Университетът / Факултети / Биологически факултет / Официални издания / Годишник на Софийския университет - КНИГА 4 Научни сесии на Биологическия факултет / Том 107, 2022 г. - Научна конференция "Климентови дни" - 2021 / - Kalina Danova et al. – BIOTECHNOLOGICAL PROPAGATION OF LOCAL MEDITERRANEAN VARIETY OF THYMUS VULGARIS L. FOR THE PURPOSE OF SECONDARY METABOLITES PRODUCTION

   

BIOTECHNOLOGICAL PROPAGATION OF LOCAL MEDITERRANEAN VARIETY OF THYMUS VULGARIS L. FOR THE PURPOSE OF SECONDARY METABOLITES PRODUCTION

KALINA DANOVA1*, ABEER YOUSRY IBRAHIM2, SABER FAYEZ HENDAWY2, MOHAMED SALAH HUSSEIN2, BORISLAVA HRISTOVA3

1 Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria
2 Medicinal and Aromatic Plants Research Department, National Research Centre, 12622, Dokki, Cairo, Giza, Egypt
3 Faculty of Chemistry and Pharmacy, Ludwig Maximilian University of Munich, 81377 Munich, Germany
* Corresponding author: k_danova@abv.bg

 

Keywords: Thymus vulgaris L. in vitro culture, phenolics in vitro, flavonoids in vitro, Thymus vulgaris L. germination, lipid peroxidation, oxidative stress

Abstract: In the present work, the potential of a local Mediterranean cultivar of Thymus vulgaris L. (ThEg) for biotechnological development was investigated. Starting material for the experiment were Thymus vulgaris L. seeds kindly provided by Sekem Company, Egypt. An experiment for comparison of the germination rate of T. vulgaris L. ThEg seeds with a commercial sample of seeds from the Bulgarian market (ThBg) was conducted in the conditions of sterile in vitro culture, as well as in commercial peat substrate propagator. In vitro shoot cultures of T. vulgaris L. of both ThEg and ThBg seeds were developed in media with modification of the auxin applied (1-Naphthaleneacetic acid vs. indole-3-butyric acid) in the presence of the cytokinin benzyl adenine. The effect of cultivation conditions on the plant‘s physiological status and polyphenolics productivity was compared showing the supplementation of 1-Naphthaleneacetic acid to be more favorable in terms of biomass formation, physiological status and polyphenolics productivity.

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