Начало / Университетът / Факултети / Биологически факултет / Официални издания / Годишник на Софийския университет - КНИГА 4 Научни сесии на Биологическия факултет / Том 105, 2020 г. - Младежка научна конференция "Климентови дни" - 2019 / - R. Ganeva, D. Parvanov, D. Nikolova, M. Vasileva, K. Nikolova, G. Stamenov - SPERM-ZONA ADHESION PROPERTIES ARE RELATED TO THE CONVENTIONAL SEMEN PARAMETERS

   

SPERM-ZONA ADHESION PROPERTIES ARE RELATED TO THE CONVENTIONAL SEMEN PARAMETERS

 

R. GANEVA*1, D. PARVANOV1, D. NIKOLOVA2, M. VASILEVA1, K. NIKOLOVA1, G. STAMENOV1

1Nadezhda Women’s Health Hospital, 3 “Blaga vest” Street, Sofia, Bulgaria
2Department of Medical Genetics, Medical Faculty, Medical University – Sofia
*Corresponding author: rum.ganeva@gmail.com

 

Abstract: The conventional semen analysis, which includes sperm volume, pH, sperm count, concentration, motility and morphology measurements, does not provide enough data on the fertilization ability of the spermatozoa. Since the sperm zona binding properties were found to be highly predictive for the assisted reproduction outcome, our aim was to estimate how the sperm-zona adhesion score (SZAS) was related to the conventional semen parameters.
A total of 141 semen samples were collected from patients of Nadezhda Women’s Health Hospital. Regular sperm analysis was performed according to WHO 5th edition (2010). Whole zonae from healthy donors’ were acid solubilised and immobilised on petri dishes. 2.5 x105 motile spermatozoa from each patient were placed in the dishes and the adhered spermatozoa were counted. Sperm-zona adhesion score (SZAS) presents the sperm-zona adhesion rate evaluated by the number of adhered spermatozoa in 1 mm2 of zona pellucida coated area.
No relation was observed between the SZAS and the physico-chemical properties of the semen (volume and pH), and the sperm count and concentration (p>0.05). The percentage of progressively motile spermatozoa and the zona adhesion rate showed slight positive correlation while the percentage of immotile spermatozoa was negatively related to the SZAS (r=0.25; P=0.03 and r=-0.16; p=0.05, respectively). The morphological evaluation also related positively to the zona adhesion ability of the spermatozoa (r=0.18; p=0.03).
In conclusion, reduced sperm mobility and morphological abnormalities may indicate dysfunctional zona adhesion properties of the spermatozoa.

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