EXPRESSION OF SPECIFIC ANTIBODIES IN NON-LYMPHOID CELL TYPES AND THEIR IMPORTANCE FOR DEVELOPMENT OF NOVEL DIAGNOSTIC, PROPHYLACTIC AND THERAPEUTIC STRATEGIES
ISKRA SAINOVA1*, VERA KOLYOVSKA1*, STEPHAN ENGIBAROV2, RUMYANA ENEVA2,
TZVETANKA MARKOVA3, DESISLAVA DRENSKA4, DIMITAR MASLAROV4
1 – Institute of Experimental Morphology, Pathology and Anthropology with Museum (IEMPAM) to Bulgarian Academy of Sciences (BAS), Sofia, Bulgaria
2 – Institute of Microbiology “Stephan Angeloff” to Bulgarian Academy of Sciences (BAS), Sofia, Bulgaria
3 – Department of Pharmacology and Toxicology to Medical University of Sofia, Bulgaria
4 – Neurology Clinic, First MHAT, Medical University of Sofia, Bulgaria
*Corresponding author: iskrasainova@gmail.com; verakol@abv.bg
Keywords: GM3 ganglioside, anti-GM3 antibodies, ELISA, immunoglobulin genes-expressing non-lymphoid cells, inter-molecular interactions
Abstract: The titers of ganglioside GM3 and anti-GM3 antibodies were assessed in extracts from brain, pancreas and in vitro-cultures of normal cells, malignant cells and mixed culture of both cell types. Total lysates from both organs were prepared (controls) and equal volumes of them were passed through GSH-Agarose Columns for separation of bio-molecules with affinity to the reduced form of tripeptide Glutathione - GSH. Separate aliquots from the lysates of both organs were mixed with lysates of laboratory-incubated cells, containing additionally-inserted copy of tumor-suppressor gene scgn, coding hormone-like protein Secretagogin (SCGN) for separation of molecules with affinity to it. The amounts of GM3 and titers of anti-GM3 antibodies in each of the prepared samples were estimated by ELISA technique, after addition of the tested ganglioside, and/or of serum, previously proved as positive on anti-GM3 antibodies. Statistically significant frequency of statistically higher GM3 and anti-GM3 antibodies titers in the lysates from cultures of malignant cells and of the mixed cultures were established, compared to the lysate from the culture of normal cells. These results were in agreement with literature data about increased titers of these molecules in neoplastic cells and malignancies. Higher titers of GM3 and anti-GM3 antibodies in the samples containing molecules with affinity to SCGN and GSH compared to the controls were assessed. The differences could be explained with decreased titers in equivalent volume of the respective biological material at the expense of other bio-molecules, not possessing such affinity. These data were in confirmation of literature findings about the ability of non-lymphoid cell types to produce immunoglobulins (antibodies). These features could suggest a possibility for development of novel strategies for diagnostic, prophylactic and therapy goals.