Начало / Университетът / Факултети / Биологически факултет / Официални издания / Годишник на Софийския университет - КНИГА 4 Научни сесии на Биологическия факултет / Том 104, 2019 г. - Международна научна конференция "Климентови дни" - 2018 / - Efrosini Taskudi, Dimitar Parvanov, Rumiana Ganeva, Biliana Hristova, Georgi Stamenov, Todor Chaushev – THE IMPACT OF STIMULATION ON VAGINAL MICROBIOTA OF WOMEN UNDERGOING IVF TREATMENT

   

THE IMPACT OF STIMULATION ON VAGINAL MICROBIOTA OF WOMEN UNDERGOING IVF TREATMENT

 

EFROSINI TASKUDI*, DIMITAR PARVANOV, RUMIANA GANEVA, BILIANA HRISTOVA,
GEORGI STAMENOV, TODOR CHAUSHEV

Nadezhda, Women’s Health Hospital, 3 “Blaga vest” Street, Sofia, Bulgaria
*Corresponding author: efrosini12@abv.bg

 

Keywords: Ovarian stimulation, vaginal microbiota, RIF patients

 

Abstract: A total of 210 patients with recurrent implantation failure (RIF) were included in the present study. 123 microbiological vaginal samples were taken from unstimulated women in regular menstrual cycle (control group) and 87 samples were taken from women during hormonal stimulation treatment (studied group). All samples were taken during the early follicular phase of the menstrual cycle. The applied culture methods for determination of dominant microorganisms in vaginal microbiota involve cultivation on sheep blood agar, MacConkey II agar, Sabouraud agar with chloramphenicol, Gardnerella selective agar, MRS agar and additional identification of gram-negative bacilli with BBL CRYSTAL™ Enteric/Nonfermenter (E/NF) Identification (ID) System. The applied ovarian stimulation did not lead to a significant change in the frequency of occurrence of Lactobacillus as a dominant species (53% vs. 50%; stimulation group vs. control group, respectively). However, we observed a dramatic increase in the percentage of cases with Gardnerella sp. (28%) in the stimulation group, which were classified as a transition to bacterial vaginosis. These findings suggest that stimulation have a negative effect on vaginal microbiota that could be a matter of concern in the consecutive IVF treatment. For this reason ovarian stimulation should be followed by vaginal microbiota recovering procedures. The use of probiotics to populate the vagina and to treat the observed infection should be considered as an optional treatment before the next IVF cycle.

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