IMMUNOHISTOCHEMICAL SEARCH FOR AGE-RELATED SENESCENT CELLS
IN HUMAN ENDOMETRIUM
DIMITAR PARVANOV*, NINA VIDOLOVA, RUMIANA GANEVA,
GEORGI STAMENOV, TODOR CHAUSHEV
Nadezhda, Women’s Health Hospital, 3 “Blaga vest” Street, Sofia, Bulgaria
*Corresponding author: dimparvanov@abv.bg
Keywords: Resveratrol, human spermatozoa, cryopreservation
Abstract: Forty five endometrial tissue sections from forty five patients with repeated implantation failure (RIF) were studied. All biopsies were taken in spontaneous cycle during the expected window of implantation (7-9 days after ovulation). Tissues were immunostained with antibodies against β-galactosidase, histone H2AX, nuclear high-mobility group box 1 (HMGB1), p16ink4a and p21. Expression of β-galactosidase, HMGB1 and H2AX was observed in a relatively high percentage of endometrial cells (92%, 58% and 39%, respectively). In contrast, p16 and p21 were positively stained in particular single cells in the endometrial stroma, glandular and surface epithelia. The p21 immunoreactivity occurred in 4.7% of the gland cells while p16 staining was observed in 9.8% of the endometrial gland cells. The overall percentage of positive p21 and p16 staining of the stromal cells was 0.6% and 3.5%, respectively. Correlation analysis revealed that the patient’s age correlated significantly only with the percentage of p21-stained cells in endometrial stroma and glands (R=0.53; p=0.002 and R=0.54; p=0.001, respectively). The obtained results show that β-galactosidase, H2AX, HMGB1 and p16 are expressed, not only by age-senescent cells but also by other cell types in endometrial tissue. Among the studied markers the most appropriate and easy detectable immunohistochemical biomarker for cellular age-associated senescence in human endometrium is p21.