Начало / Университетът / Факултети / Биологически факултет / Официални издания / Годишник на Софийския университет - КНИГА 4 Научни сесии на Биологическия факултет / Том 102, 2017 г. - Младежка научна конференция "Климентови дни" - 2016 г. / Velmira Dimitrova, Teodora Georgieva, Maria Geneva, Yuliana Markovska - DETERMINATION OF TOTAL ANTIOXIDANT CAPACITY IN TWO LYCIUM SPECIES GROWN EX VITRO AT HIGH SALINITY IN HYDROPONIC

   

DETERMINATION OF TOTAL ANTIOXIDANT CAPACITY IN TWO LYCIUM SPECIES GROWN EX VITRO AT HIGH SALINITY IN HYDROPONIC

 

VELMIRA DIMITROVA1,2*, TEODORA GEORGIEVA2, MARIA GENEVA3, YULIANA MARKOVSKA1

 

1–Department of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Sofia, Bulgaria

2–Biotree LTD, Sofia, 7 Banskoshosestr.,Sofia, Bulgaria

3–Department of Plant Soil Interactions, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev str., Bl.21, Sofia, Bulgaria

*Corresponding author:velmi@abv.bg

 

Keywords: Lycium barbarum, Lycium chinense, K, Na distribution, leaf antioxidant capacity

 

Abstract: The effect of salt stress on distribution of alkaline metals in the roots and shoots of Lycium barbarum and Lycium chinense grown ex vitro in hydroponic at three levels of salinity, 0.05 M, 0.1 M and 0.2 M NaCl was investigated. The content of Na+ and K+ gradually decreased in the roots and rose in the shoots of Lycium chinense with increasing salinity level as compared to control. The content of Na+ increased sharply in the roots, but increase in the leaves of Lycium barbarum was slight. The ratios K+/Na+ were higher in the shoots in comparison to roots of both plants. They were reduced more in the roots of L. barbarum and in the shoots of L. chinense during NaCl treatment. Total antioxidant capacity, measured by FRAP assay and DPPH radical methods changed by the same manner in the leaves of L. chinense and were enhanced after treatment with 50 mM NaCl. The results were discussed from the view of mechanisms of action of alkaline metals on the generation of reactive oxygen species (ROS) in different organs and the participation of water soluble (WS-AOC) and lipid soluble (LS-AOC) antioxidants in the defence of plants. WS-AOC was higher and total DPPH and FRAP antioxidant capacity measured was greater in the leaves of Lycium chinense.

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