Начало / Университетът / Факултети / Биологически факултет / Официални издания / Годишник на Софийския университет - КНИГА 4 Научни сесии на Биологическия факултет / Том 106, 2021 г. - Научна конференция "Климентови дни" - 2020 / - Kalina Danova, Vaclav Motyka, Petre Dobrev - COULD EVOLUTIONARY FACTORS AFFECT ENDOGENOUS CYTOKININ POOLS IN HYPERICUM SPECIES IN VITRO?

   

COULD EVOLUTIONARY FACTORS AFFECT ENDOGENOUS CYTOKININ POOLS IN HYPERICUM SPECIES IN VITRO?

KALINA DANOVA1*, VACLAV MOTYKA2, PETRE DOBREV2

1Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria, k_danova@abv.bg
2Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, Prague, Czech Republic, Motyka@ueb.cas.cz

 

*Corresponding author: k_danova@abv.bg

 

Keywords: Hypericum in vitro culture, Hypericum calycinum, Hypericum perfortum, Hypericum tetrapterum, Hypericum richeri, endogenous cytokinins in vitro

 

Abstract: In the present work, shoot cultures of hypericin non-producing Hypericum calycinum L. (of the primitive Ascyreia section), hypericin producing H. perforatum L. and H. tetrapterum Fr. (both section Hypericum) and H. richeri Vill. (the evolutionary most advanced Drosocarpium section in our study) were developed. For the purpose of multiplication stimulation, cytokinin (CK) N6-benzyladenine and auxin indole-3-butyric acid were exogenously applied in different combinations. The content of total endogenous CKs were studied and their pools and ratios between cis- and trans-zeatin types were compared.

FULL TEXT